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GAHK20
Sigma
Glucose (HK) Assay Kit
sufficient for 20 assays
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration. |
Properties
| usage | sufficient for 20 assays |
| storage temp. | 2-8°C |
Safety
Components
| Standard component | glucose (HK) assay reagent 20 ml |
| glucose standard 0.5 mL |
References
| reference | Kunst, A., et al. Meth. Enzym. Anal. 3rd ed.,, 163-172, (1984) |
